Summary: This protocol details the isolation ProLogic Parts and in vitro maintenance of single hematopoietic stem cells (HSCs) in the absence of the bone marrow niche.The HSCs do not divide over 7 days and fully retain their long-term functional capacity in transplantation assays.Following hibernation culture, HSCs can be used to study quiescence S

It has been shown that gene body DNA methylation is associated with gene expression.However, whether and how deviation of gene body DNA methylation between duplicate genes can influence their divergence remains largely unexplored.Here, we aim to elucidate the potential role of gene body DNA methylation in the fate of duplicate genes.We identified p

Abstract This Critical Synthesis Package contains: (1) a Critical Analysis of the psychometric properties and the application to health science education of the Index for Interdisciplinary Collaboration (IIC), and (2) Bang a copy of the IIC developed by Laura R.Bronstein, LCSWR, ACSW, PhD.The IIC was designed to measure the perception of collaborat

Abstract: This paper is structured to provide a description of the planning of gender responsive early childhood programs.The hope is that gender responsive early childhood education programs can shape and develop a gender equitable early childhood outlook.This can be done by preparing a gender responsive early childhood program planning.There are

Passenger cars require different types of periodic maintenance.The most common and well-known procedure is the replacement of LIQUID ACTIVATOR oil and filters, the so-called basic service.At the time of a pandemic, there is a need for social distance, reduced interaction between people and avoidance of all possible contamination of personal belongi